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The mutants were analyzed by inverse PCR, utilizing two oligonucleotides, OTn1 and OTn2, to amplify adjacent DNA fragment to the transposon insertion website, followed by sequencing in the cloned solution (see Materials and Approaches
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Artinya, tanaman padi itu harus berasal dari bibit first, karena apabila hasil panen kemudian ditanam ulang, hasil ini akan berbeda dengan bibit aslinya. Harga benih varietas hibrida ini termasuk yang termahal.
I, and religated, along with the round DNA was used as matrix for PCR amplification. The DNA fragments So amplified ended up instantly cloned in the PCR2.one vector With all the TA cloning package (Invitrogen) and transformed into E. coli
mutants faulty in distinctive phases of biofilm development, a number of elements that get involved in the initiation of biofilm formation and progression to microcolonies and experienced biofilm ended up discovered (9). Through the Evaluation from the phenotypes of different P. aeruginosa
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Tanda-tanda bahwasanya tanaman padi akan siap dipanen adalah dengan menguningnya warna dari kulit bulir padi dan juga tangkai padi secara menyeluruh.
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